Alternative splicing regulation appears to play a crucial role in grape berry development and is also potentially involved in adaptation responses to the environment

Table 3 Successive up- and down-regulation of a Gw-specific IR event in the 3’UTR of CALR3

				IL - Gw			ILD - Gw
Gene ID^a	GENE NAME – biological process/function	AS^b (region)^c	specificity	S1	S2	S3	S1 vs S2 (FDR)	S2 vs S3 (FDR)
Vitvi04g01198	*CALR3* - unfolded protein response	IR (3’UTR)	ILs-Ri = 1	0.47	0.68	0.49	−0.22 (0)	0.19 (0)

The IR event was first increased between S1 (green berry at 6 weeks post-flowering) and S2 (hard berry at mid-véraison), then was diminished between S2 and S3 (soft berry at mid-véraison). The values of IL and ILD (significant at FDR ≤ 0.05) were means of three biological replicates
^aaccording to the VCost.v3 genome annotation
^bAS type, comprising A3SS, A5SS, ES and IR events
^cregion affected by the AS event (3′-UTR, 5′-UTR or CDS)

ISSN: 1471-2229