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Table 4 AS events regulated between véraison and mid-ripening in only one variety

From: Alternative splicing regulation appears to play a crucial role in grape berry development and is also potentially involved in adaptation responses to the environment

    

IL - Gw

ILD - Gw

IL - Ri

ILD - Ri

Gene IDa

GENE NAME – biological process/function

ASb (region)c

specificity

S3

S4

S3 vs S4 (FDR)

S3

S4

S3 vs S4 (FDR)

Similar isoform ratios between Gw and Ri before mid-ripening

 Vitvi01g00135

VPS52 - intracellular trafficking

IR-1 (5’UTR)

_

0.27

0.12

0.14 (3.2 E-03)

_

_

_

 Vitvi01g00135

VPS52 - intracellular trafficking

IR-2 (5’UTR)

_

_

_

_

0.54

0.76

−0.22 (1.1 E-02)

 Vitvi04g01976

RSZ22 - splicing regulation

ES (5’UTR)

_

_

_

_

0.32

0.15

0.18 (2.8 E-10)

 Vitvi05g00214

BOR3 - boron transport

A3SS (CDS)

_

_

_

_

0.77

0.52

0.25 (5.9 E-06)

 Vitvi05g00217

RH40 - NMD and ribosome biogenesis

A3SS (CDS)

_

_

_

_

0.42

0.22

0.20 (1.8 E-02)

 Vitvi09g01457

ECT5 - mRNA binding protein

A3SS (5’UTR)

_

_

_

_

0.54

0.77

−0.24 (2.0 E-02)

 Vitvi13g00175

UXT3 - xylose transport

IR (5’UTR)

_

_

_

_

0.24

0.44

−0.20 (7.6 E-04)

 Vitvi13g00613

PHOSPHOGLUCOSAMINE MUTASE - carbohydrate metabolism

ES (CDS)

_

_

_

_

0.55

0.25

0.29 (2.5 E-07)

 Vitvi18g01662

STOP1 - response to acidic stress

ES (5’UTR)

_

_

_

_

0.09

0.34

−0.25 (1.8 E-07)

 Vitvi18g00072

PP2C27 - abiotic stress modulation

IR-1 (5’UTR)

_

0.78

0.52

0.26 (1.5 E-05)

_

_

_

 Vitvi18g00072

PP2C27 - abiotic stress modulation

IR-2 (5’UTR)

_

0.88

0.73

0.15 (7.9 E-03)

_

_

_

Differential isoform ratios before mid-ripening

 Vitvi01g00819

NLRC3 - intracellular signal transduction

IR (CDS)

ILs-Gw = 1

_

_

_

0.47

0.86

−0.40 (6.7 E-04)

 Vitvi01g02098

SIN3A ISOFORM G - transcription regulator

A3SS (3’UTR)

_

_

_

_

0.96

0.79

0.17 (5.9 E-05)

 Vitvi02g00597

CKB1 - regulation of protein kinase activity

A5SS (CDS)

_

_

_

_

0.54

0.81

−0.27 (0.0 E+00)

 Vitvi06g01662

S1FA1 - regulation of transcription

A3SS (5’UTR)

_

_

_

_

0.07

0.54

−0.48 (6.7 E-03)

 Vitvi13g01915

AIMP1 - tRNA aminoacylation

A5SS (CDS)

ILs-Gw = 1

_

_

_

0.65

0.91

−0.26 (1.2 E-09)

 Vitvi13g02138

ARPP21 - calmodulin-binding

ES (5’UTR)

_

0.55

0.74

−0.20 (1.3 E-02)

_

_

_

 Vitvi14g00256

Unknown

ES (CDS)

_

_

_

_

1.00

0.58

0.41 (2.1 E-02)

 Vitvi14g02541

PROBABLE LIGASE

ES (CDS)

ILs-Gw = 1

_

_

_

0.60

0.96

−0.36 (6.9 E-12)

 Vitvi15g00621

p53 - DNA damage repair

IR (3’UTR)

ILs-Gw ≤ 0.02

_

_

_

1.00

0.81

0.19 (2.3 E-02)

 Vitvi15g01145

AHL9 - DNA-binding transcription factor

A5SS (5’UTR)

_

0.80

0.95

−0.16 (8.9 E-05)

_

_

_

 Vitvi15g01145

AHL9 - DNA-binding transcription factor

A3SS (5’UTR)

_

0.80

0.95

−0.15 (2.8 E-03)

_

_

_

 Vitvi15g01145

AHL9 - DNA-binding transcription factor

IR (5’UTR)

_

0.88

0.97

−0.09 (1.6 E-02)

_

_

_

 Vitvi15g01173

C1-THF synthase - folate synthesis

ES (CDS)

_

0.67

0.85

−0.19 (1.0 E-03)

_

_

_

 Vitvi17g00038

CLPC1 - protein translocation to chloroplast

A3SS (5’UTR)

_

_

_

_

1.00

0.78

0.22 (1.1 E-02)

 Vitvi17g00770

WNK - response to stress

A3SS (CDS)

ILs-Gw ≤ 0.02

_

_

_

0.58

0.27

0.31 (1.0 E-02)

  1. The regulation occurred between véraison (S3: soft berry at mid-véraison) and mid-ripening (S4: mid-ripening). Similar or differential isoform ratios were observed between Gw and Ri before mid-ripening; ILDs between Gw and Ri at the different stages are given in the Additional file 4
  2. aaccording to the VCost.v3 genome annotation
  3. bAS type, comprising A3SS, A5SS, ES and IR events
  4. cregion affected by the AS event (3′-UTR, 5′-UTR or CDS)