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Table 5 AS events showing differential isoform ratios between Gw and Ri with no significant regulation between stages

From: Alternative splicing regulation appears to play a crucial role in grape berry development and is also potentially involved in adaptation responses to the environment

    

ILD – Gw vs Ri

Gene IDa

GENE NAME – biological process/function

ASb (region)c

specificity

S1 (FDR)

S2 (FDR)

S3 (FDR)

S4 (FDR)

Gw-specific or -preferential events

 Vitvi02g00123

DSPP - calcium ion binding

A3SS (5’UTR)

ILs-Ri = 1

−0.43 (0)

−0.40 (0)

−0.39 (0)

−0.35 (0)

 Vitvi03g01316

NUP160 - nucleus-to-cytoplasm mRNAs transfer

IR (3’UTR)

ILs-Ri ≥ 0.97

−0.47 (0)

−0.42 (0)

−0.43 (0)

weak expression

 Vitvi04g01192

KRI1 - DNA damage-induced cell apoptosis

ES (3’UTR)

ILs-Ri ≤ 0.01

0.36 (9.1 E-10)

0.23 (1.4 E-05)

0.16 (3.7 E-04)

0.16 (2.6 E-05)

 Vitvi04g01520

GDAP2 - growth and development

IR (3’UTR)

ILs-Ri = 1

−0.62 (0)

−0.52 (0)

−0.56 (0)

−0.52 (0)

 Vitvi05g01796

MEE - development regulation

ES (5’UTR)

ILs-Ri ≥ 0.97

−0.53 (0)

−0.50 (0)

−0.49 (0)

−0.33 (4.5 E-05)

 Vitvi06g00189

SAP5 - environmental stress response

A5SS (5’UTR)

ILs-Ri ≤ 0.04

0.40 (0)

0.37 (0)

0.32 (0)

0.29 (0)

 Vitvi12g00120

ACD32.1 - stress response

A5SS (CDS)

ILs-Ri ≤ 0.01

0.30 (0)

0.32 (0)

0.27 (0)

0.28 (0)

 Vitvi12g00210

unknown

A3SS (CDS)

ILs-Ri = 0

weak expression

0.37 (1.0 E-07)

0.50 (2.5 E-14)

0.30 (1.1 E-12)

 Vitvi12g02216

NRPB4 - transcription initiation

IR (3’UTR)

ILs-Ri = 0

0.59 (0)

0.62 (0)

0.57 (0)

0.53 (0)

 Vitvi14g01289

SAMDC2 - polyamines biosynthesis/homeostasis

ES (5’UTR)

ILs-Ri = 0

0.58 (0)

0.51 (0)

0.55 (0)

0.59 (0)

 Vitvi14g02028

GAUT 13 - pectin/xylans biosynthesis

ES (CDS)

ILs-Ri = 1

−0.23 (4.2 E-07)

−0.17 (3.8 E-04)

−0.24 (0)

−0.15 (9.0 E-12)

 Vitvi15g00615

HIBCH1 - amino acid catabolic process

A5SS (CDS)

ILs-Ri ≥ 0.98

−0.34 (4.5 E-12)

−0.36 (3.6 E-13)

−0.34 (5.6 E-10)

weak expression

 Vitvi15g01132

FCF1 - rRNA-processing & 40S assembly

ES (5’UTR)

ILs-Ri ≥ 0.99

−0.15 (8.7 E-04)

−0.14 (3.6 E-04)

−0.18 (1.7 E-05)

−0.16 (ns)

 Vitvi15g01132

FCF1 - rRNA-processing & 40S assembly

ES (5’UTR)

ILs-Ri ≥ 0.98

−0.15 (1.1 E-05)

−0.17 (7.4 E-06)

−0.20 (1.4 E-07)

−0.29 (1.9 E-05)

Ri-specific or -preferential events

 Vitvi01g01659

RANBP2 - mRNA processing

ES (CDS)

ILs-Gw ≥ 0.98

weak expression

0.15 (6.7 E-04)

0.28 (0)

0.14 (1.5 E-03)

 Vitvi02g00836

XLG3 - receptor signaling pathway

IR (5’UTR)

ILs-Gw ≥ 0.99

0.34 (0)

0.30 (1.1 E-11)

0.28 (8.7 E-10)

0.36 (1.3 E-09)

 Vitvi03g01648

Unknown

A3SS (5’UTR)

ILs-Gw ≤ 0.02

−0.49 (0)

−0.70 (0)

−0.50 (0)

−0.42 (0)

 Vitvi04g01559

c(3)G - DNA double-strand breaks repair

A3SS (5’UTR)

ILs-Gw ≥ 0.99

0.39 (0)

0.36 (0)

0.41 (1.9 E-12)

0.45 (0)

 Vitvi04g02261

MPPED2 - unknown

ES (CDS)

ILs-Gw ≥ 0.99

0.75 (0)

0.85 (0)

0.86 (0)

0.64 (0)

 Vitvi08g01204

PBL11, NAK - growth and development

A3SS-1 (5’UTR)

ILs-Gw = 1

0.54 (6.7 E-14)

0.46 (0)

0.47 (ns)

weak expression

 Vitvi08g01204

PBL11, NAK - growth and development

A3SS-2 (5’UTR)

ILs-Gw = 1

0.42 (ns)

0.28 (1.7 E-06)

0.47 (ns)

weak expression

 Vitvi11g00574

FBL14 - ubiquitin-dependent protein catabolism

A3SS (5’UTR)

ILs-Gw = 1

0.22 (0)

0.25 (0)

0.20 (0)

0.26 (0)

 Vitvi11g00614

EF1B - translation elongation

A3SS (5’UTR)

ILs-Gw = 1

0.34 (9.7 E-12)

0.36 (3.9 E-11)

0.49 (1.3 E-12)

weak expression

 Vitvi11g00614

EF1B - translation elongation

ES (5’UTR)

ILs-Gw ≥ 0.97

0.54 (0)

0.36 (2.0 E-03)

0.57 (0)

0.77 (0)

 Vitvi11g01128

GCNT – transfer of glycosyl groups

A3SS (5’UTR)

ILs-Gw ≤ 0.02

−0.27 (4.1 E-14)

−0.34 (6.9 E-12)

−0.31 (2.2 E-10)

weak expression

 Vitvi12g02011

RPPL1 - disease resistance

IR (3’UTR)

ILs-Gw ≥ 0.93

0.34 (3.0 E-04)

0.32 (2.2 E-05)

0.41 (3.1 E-14)

0.42 (4.3 E-12)

 Vitvi14g01605

G6PD - glucose metabolic process

ES (5’UTR)

ILs-Gw = 0

−0.16 (0)

−0.18 (1.6 E-13)

−0.18 (0)

−0.18 (0)

 Vitvi15g00615

HIBCH1 - branched-chain amino acid catabolism

ES (CDS)

ILs-Gw = 1

0.29 (3.7 E-10)

0.31 (5.7 E-10)

0.27 (1.4 E-06)

weak expression

Other differential events

 Vitvi01g00383

MAF1 - repression of transcription

A3SS (5’UTR)

Isoform shift

0.44 (0)

0.45 (0)

0.43 (1.2 E-13)

0.45 (3.3 E-10)

 Vitvi03g00257

ACY1 - hydrolysis of N-acylated amino acids

A3SS (CDS)

_

0.16 (0)

0.16 (0)

0.16 (0)

0.14 (9.5 E-06)

 Vitvi06g00073

SCL30A - regulation of mRNA splicing

ES (CDS)

Isoform shift

weak expression

0.27 (ns)

0.32 (6.2 E-03)

weak expression

 Vitvi06g00216

DTX42 - aluminium tolerance

A5SS (5’UTR)

Isoform shift

0.56 (0)

0.39 (9.7 E-10)

0.42 (1.9 E-04)

0.30 (1.3 E-03)

 Vitvi07g01499

GCL1 - carbohydrate metabolic process

ES (CDS)

_

−0.30 (1.4 E-07)

−0.28 (2.0 E-04)

−0.24 (1.9 E-09)

−0.32 (0)

 Vitvi09g01389

PUM5 - regulation of translation

A3SS (3’UTR)

Isoform shift

weak expression

0.31 (2.3 E-05)

0.24 (1.2 E-03)

0.29 (4.5 E-05)

 Vitvi10g00052

OSCA1 - response to osmotic stress

A5SS (5’UTR)

Isoform shift

−0.30 (1.1 E-08)

−0.25 (8.5 E-04)

−0.27 (1.0 E-04)

−0.35 (0)

 Vitvi10g00094

SS3 - starch biosynthesis

A5SS (5’UTR)

_

−0.22 (1.5 E-03)

−0.27 (5.7 E-04)

−0.28 (1.4 E-05)

−0.21 (ns)

 Vitvi10g00577

HYDROLASE-LIKE PROTEIN FAMILY

A5SS (3’UTR)

Isoform shift

0.29 (1.0 E-02)

0.21 (ns)

0.32 (7.5 E-04)

0.32 (6.9 E-03)

 Vitvi11g00473

RIBOSOMAL PROTEIN - translation

ES (5’UTR)

_

0.13 (1.9 E-03)

0.20 (2.5 E-13)

0.16 (5.9 E-09)

0.14 (4.1 E-05)

 Vitvi15g00926

RS40 – RNA/mRNA splicing

ES (5’UTR)

Isoform shift

0.34 (3.0 E-03)

0.23 (ns)

0.34 (3.6 E-10)

0.23 (1.7 E-06)

  1. AS events were split in variety-specific or -preferential events (Gw or Ri) and other differential events. The examined stages were S1 (green berry at 6 weeks post-flowering), S2 (hard berry at mid-véraison), S3 (soft berry at mid-véraison) and S4 (mid-ripening). The value of ILD (significant at FDR ≤ 0.05) was a mean of three biological replicates
  2. aaccording to the VCost.v3 genome annotation
  3. bAS type, comprising A3SS, A5SS, ES and IR events
  4. cregion affected by the AS event (3′-UTR, 5′-UTR or CDS)