The Protein Disulfide Isomerase gene family in bread wheat (T. aestivum L.)

BMC Plant Biology

Table 3 Characteristics of the wheat PDI-like proteins.

Name	Length	Mw	pI	N-glycisilation sites (putative)	Domain composition^a	Active site sequence	Conserved charge pair sequence^b	Conserved arginine^b
TaPDIL1-1a	515	56.59	4.99	1: N283	a-b-b'-a'	CGHC, CGHC	E62-K96, E406-K439	R136, R475
TaPDIL1-1b	512	56.44	5.03	1: N283	a-b-b'-a'	CGHC, CGHC	E62-K96, E406-K439	R136, R475
TaPDIL1-1c	515	56.63	4.96	1: N283	a-b-b'-a'	CGHC, CGHC	E62-K96, E406-K439	R136, R475
TaPDIL2-1	588	63.80	4.61	2: N109, N212	c-a-b-b'-a'	CGHC, CGHC	E123-K157, E464-K497	R193, R535
TaPDIL3-1	541	59.61	4.95	1: N150	c-a-b-b'-a'	CERS, CVDC	L90-K124, E429-R462	R160, O492
TaPDIL4-1a	367	40.27	6.17	0	a°-a-D	CGHC, CGHC	E54-K87, E173-K211	R125, R244
TaPDIL4-1b	367	40.26	6.17	0	a°-a-D	CGHC, CGHC	E54-K87, E173-K211	R125, R244
TaPDIL5-1a	440	47.15	5.12	1: N170	a°-a-b	CGHC, CGHC	E51-K89, E188-K226	R119, R257
TaPDIL5-1b	440	47.22	5.36	1: N170	a°-a-b	CGHC, CGHC	E51-K89, E188-K226	R119, R257
TaPDIL6-1a	151	16.99	4.96	0	a	CKHC	Q56-S95	R126
TaPDIL6-1b	149	16.65	5.30	0	a	CKHC	Q54-S93	R124
TaPDIL7-1a	413	46.30	4.91	1: N275	a-b-b'-t	CGHC	D56-K90	R126
TaPDIL7-1b	417	46.62	4.91	2: N176, N279	a-b-b'-t	CGHC	D60-K94	R130
TaPDIL7-1c	413	46.32	4.87	2: N172, N275	a-b-b'-t	CGHC	D56-K90	R126
TaPDIL7-2a	418	46.40	5.12	0	a-b-b'-t	CGHC	D64-K98	R134
TaPDIL7-2b	418	46.34	5.03	1: N384	a-b-b'-t	CGHC	D64-K98	R134
TaPDIL8-1	485	54.41	6.90	ND	t-a-t	CYWS	N164-K203	R249

^a a = active site containing thioredoxin-like domain; b = inactive thioredoxin-like domain (note that the superscript are included to distinguish between domains of proteins containing more than one a and b domain on the basis of their position and not on the basis of sequence homology); c = acidic segment; D = Erp29c domain; t = transmembrane domain. ^b The position of conserved charge pair sequence and arginine residues that are considered important for the catalytic activity of different members of the human PDI family are determined on the basis of multiple alignments of the atype domains of wheat PDI-like proteins and human classical PDI [Accession number P07237] (Figure 3). ND = not determined because TaPDIL8-1 lacks a putative N-terminal signal peptide. Proteins without signal peptides are unlikely to be exposed to N-glycosilation machinery and thus may not be glycosylated in vivo even though they contain potential motifs.

ISSN: 1471-2229