Figure 2

A phylogenetic tree inferred using CLUSTAL W from the deduced amino acid sequences for F3'5'H , F3'H and C4H. F3'5'H (mauve shading), F3'H (red shading), C4H (cinnamate 4-hydroxylase, no shading), a less closely related sequence for a cytochrome P450 enzyme involved in flavonoid biosynthesis. The phylogenetic tree shows bootstrap values. The F3'5'H sequences form a distinct cluster, which includes the cyclamen sequence. Based on the amino acid and phylogeny analysis the evidence supports CpF3'5'H as encoding F3'5'H.