Differential expression of maize chitinases in the presence or absence of Trichoderma harzianum strain T22 and indications of a novel exo- endo-heterodimeric chitinase activity

Table 1 Identification of chitinases from the activity bands.

Plant treatment	Tissue	Protein treatment	Band	Identity	% Confidence
Control	Shoot	55°C	1	Exo2; chiIVA	96; 99
			2	No chitinase identified
			3	No chitinase identified
			4	chiIVB	99
			5	ChiI67336	99
Trichoderma	Shoot	55°C	1	Exo2; ChiIII9675	96; 96
			2	No chitinase identified
			3	chiI11654	99
			4	No chitinase identified
			5	ChiI67336	99
Control	Roots	55°C	6	Exo2; ChiIII9615	96; 98
			8	No chitinase identified
Trichoderma	Roots	55°C	6	Exo2	90
			8	No chitinase identified
Control	Roots	Boiling	7	chiIII9615	97
Trichoderma	Roots	Boiling	7	chiIII9615	99

Bands harboring chitinase activity were cut from gels and sent for identification by LC/MS/MS. The plant treatment and the tissues from which the proteins were extracted are listed. The proteins were either heated to 55°C or boiled before loading on gel. The numbers of the bands match the activity bands marked in Figure 1. The chitinases identified and the level of confidence is listed (when two proteins identified their % of confidence is listed respectively). The amino acid sequences, binding domains and other information for each of the proteins is described (Shoresh and Harman, 2008a).

ISSN: 1471-2229