Figure 3From: A new allele of flower color gene W1 encoding flavonoid 3'5'-hydroxylase is responsible for light purple flowers in wild soybean Glycine sojaHPLC chromatogram of anthocyanins extracted from flower petals of PI 424008A (upper panel) and B09121 (lower panel). 200 mg of banner petals was extracted with 2 ml of MeOH containing 0.1% HCl. Eluents: MeCN/HOAc/H2O/H3BO3 (6:8:83:3). Flow-rate: 1.0 ml/min. Injection: 10 μl. Detection: 530 nm, A1 = malvidin 3,5-di-O-glucoside, A2 = petunidin 3,5-di-O-glucoside, A3 = delphinidin 3,5-di-O-glucoside, A4 = delphinidin 3-O-glucoside, A5 = peonidin 3,5-di-O-glucoside, A6 = cyanidin 3,5-di-O-glucoside, A7 = cyanidin 3-O-glucoside.Back to article page