Figure 4
Results of dCAPS and indel analysis of F3'5'H gene in a soybean cultivar Clark, a near-isogenic line of Clark with white flowers, Clark- w1 and a Glycine soja line with light purple flowers, B09121. (Left panel) Results of dCAPS analysis. PCR products amplified with dCAPS primers were digested by NcoI and the digests were separated on an 8% polyacrylamide gel. Ï•, molecular marker Ï•x174/HaeIII; clk, Clark; w1, Clark-w1; B, B09121. The migration of size markers is shown to the left of the gel. (Right panel) Results of indel analysis. PCR products amplified with indel primers were separated on an 8% polyacrylamide gel. Ï•, molecular marker Ï•x174/HaeIII; clk, Clark; w1, Clark-w1; B, B09121.