Figure 5From: Diverse accumulation of several dehydrin-like proteins in cauliflower (Brassica oleracea var. botrytis), Arabidopsis thaliana and yellow lupin (Lupinus luteus) mitochondria under cold and heat stressWestern analysis of dehydrin-like proteins in yellow lupin mitochondria. Dlps were analyzed in mitochondrial extracts from imbibed seeds (S) and seedling hypocotyls (Hy) of yellow lupin and from the mitochondria of Arabidopsis cell cultures (Ara). The lupin imbibed seeds and seedlings hypocotyls were grown under control conditions (1), or subjected to cold (2) or heat (3) treatment. Ten micrograms of mitochondrial proteins were loaded per lane. Western analysis was carried out using the antibody against K-segment of dehydrins (@K), the same antibody blocked with peptide (@K + peptide) and the antibody against DHN24 dehydrin (@DHN24). The representative Western blots came from 3 experimental repetitions.Back to article page