Figure 6

CrSGD is localised to the nucleus of C. roseus cells with a diffuse or a punctuated/fusiform aggregation-like pattern of fluorescence dependant on the orientation of the GFP fusion. Undifferentiated C. roseus cells were co-transformed with CrSGD-GFP or GFP-CrSGD constructs (1^st column) together with mcherry or YFP organelle markers constructs (2^nd column). The merged image and the DIC morphology are presented in the 3^rd and 4^th columns, respectively. Note that SGD fusions were localised to the nucleus and not to the ER with either a diffuse fluorescence pattern (CrSGD-GFP) and a punctuated or fusiform aggregation-like pattern of fluorescence (GFP-CrSGD). Bar: 10 μm.