Figure 9

The subcellular localisation of STR and SGD isolated from R. serpentina is conserved but the RsRGD does not aggregate in the nucleus. Undifferentiated C. roseus cells were co-transformed to express RsSTR, RsSGD and RsRGD GFP fusions (1^st column) and a nucleus marker (2^nd column). The merged image and the DIC morphology are presented in the 3^rd and 4^th columns, respectively. Note that both RsSTR and RsSGD displayed exactly the same fluorescence patterns as CrSTR and CrSGD, respectively. Note also that RsRGD-GFP and GFP-RsRGD displayed a diffuse nuclear fluorescence pattern reinforcing the specificity of the aggregation of CrSGD and RsSGD. Bar: 10 μm.