Figure 2
The seu cyp85A2 double mutants condition enhanced outer integument defects. Photomicrograph (Nomarski optics) of chloral hydrate cleared ovules of the indicated genotypes. Extent of inner (ii) and outer (oi) integument growth is indicated. A) Col-0 wild type. B and C) cyp85A2-1 and cyp85A2-2 ovules display near wild type morphology. D) seu-3 single mutant displays slightly reduced growth of outer integument. E) seu cyp85A2-2 ovule. Disruption of outer integument development is greater in the double mutant relative to wild type or either single mutant. F-H) Three examples of seu cyp85A2-1 double mutants display varying degrees of outer integument disruption, but are consistently more disrupted than the wild type or single mutants. Scale bar in H is 100 microns for all panels.