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Table 1 Programs used to predict trans-membrane domain of ICMEs of Arabidopsis

From: Characterization, sub-cellular localization and expression profiling of the isoprenylcysteine methylesterase gene family in Arabidopsis thaliana

Programs and their websites

Proteins

No. of transmembrane domains

DAS

http://www.sbc.su.se/~miklos/DAS/

ICME-LIKE1

6 (loosing cutoff 1.7 ); 2 (strict cutoff 2.2)

 

ICME-LIKE2

4 (loosing cutoff 1.7 ); 4 (strict cutoff 2.2)

 

ICME

4 (loosing cutoff 1.7 ); 2 (strict cutoff 2.2)

HMMTOP

http://www.enzim.hu/hmmtop/html/submit.html

ICME-LIKE1

2 (N-terminus: OUT)

 

ICME-LIKE2

4 (N-terminus: IN)

 

ICME

4 (N-terminus: IN)

SOSUI

http://bp.nuap.nagoya-u.ac.jp/sosui/sosui_submit.html

ICME-LIKE1

2

 

ICME-LIKE2

2

 

ICME

NO

TMpred

http://www.ch.embnet.org/software/TMPRED_form.html

ICME-LIKE1

4 (Inside to outside helices);5 (Outside to inside helices)

 

ICME-LIKE2

5 (Inside to outside helices);5 (Outside to inside helices)

 

ICME

5 (Inside to outside helices);7 (Outside to inside helices)

TMHHM

http://www.cbs.dtu.dk/services/TMHMM-2.0/

ICME-LIKE1

1 (N-terminus: IN)

 

ICME-LIKE2

1 (N-terminus: IN)

 

ICME

1 (N-terminus: IN)