Figure 4

Yeast complementation analysis of TaPMM genes. Five yeast constructs, harboring the cDNA of TaPMM-A1 (p181-A1), B1 (p181-B1), D1 (p181-D1), B2 (p181-B2) or D2 (p181-D2), were prepared using the expression vector p181AINE (containing a Leu2 selection marker). These constructs, together with the empty vector control (p181-VC), were transformed into the sec53-6 strain of S. cerevisiae, which does not grow at elevated temperatures (30°C or above) because of temperature sensitive mutation in the native PMM. The six recombinant strains, sec53-6, and RSY249 (the wild type progenitor of sec53-6) were serially diluted and spotted onto the medium lacking leucine, followed by incubation at three test temperatures (24, 30 or 37°C), The data shown are reproducible in three independent experiments.