Figure 2

FAD2 and FAE1 protein alignment. (A) Amino acid sequence comparison of the three Camelina sativa FAD2 sequences, Arabidopsis thaliana FAD2 sequence [Genbank: NP_187819], Brassica rapa FAD2 sequence [Genbank: AJ459107], Glycine max FAD2-3 sequence [Genbank: DQ532371], Zea mays FAD2 sequence [Genbank: AB257309]. Blue underlines below the sequences indicate amino acids conserved in all 50 FAD2 sequences compared by Belo et al.[46] while the green underline indicates the ER localization signal [45]. The three His boxes described by Tocher et al [44] are indicated with red boxes. (B) Amino acid sequence comparison of the three Camelina sativa FAE1 sequences, Arabidopsis thaliana FAE1 [Genbank:NP_195178], Crambe abyssinica [Genbank: AAX22298], Brassica rapa Heac FAE1 [Genbank: Y14975], Brassica rapa Leac FAE1 [Genbank: Y14974], Limnanthes alba (meadow foam) [Genbank: AF247134] and Tropaeolum majus (nasturtium) [Genbank: ABD77097]. Blue underlines below the sequence indicate the asparagine at position 424 and the highly conserved histidine and cysteine residues described by Ghanevati and Jaworski [49, 50]. The red box indicates the region highly conserved among condensing enzymes in very long chain fatty acid biosynthesis [62] Abbreviations: Heac = High erucic acid, Leac = Low erucic acid.