Yemenite accessions 5589 and 5590 harbor the natural mlo -11 allele. Genomic DNA of the indicated barley lines was used as a template for PCR amplification (40 cycles; extension 1 minute at 72°C) using either the oligonucleotide combination ADUP7A/Mlo6 (diagnostic for the presence of the mlo-11 repeat structure ) or Mlo6/Mlo10 (indicating presence/absence of a MITE associated with the 5'-terminal repeat in the majority of mlo-11 haplotypes [11, 30]). PCR products were separated by agarose gel electrophoresis and visualized via ethidium bromide staining in combination with UV transluminescence. Cultivar Ingrid (Mlo genotype) and line back cross Ingrid (BCI) mlo-11 served as controls for wild type and mlo-11 genotypes, respectively. Note that lines RAH995 and Ab1089 were included as additional controls.