Figure 5
A map of amino acid residues that are critical for Mlo function in the context of powdery mildew susceptibility. Schematic representation of the barley Mlo protein and the relative position of loss-of-function single amino acid substitution mutations and a two amino acid deletion mutant (mlo-10). The light grey horizontal rectangle depicts the lipid bilayer, the grey vertical rectangles the seven transmembrane domains and the black bends the extracellular and cytoplasmic loops. NH2 and COOH denote the amino and carboxyl-terminus, respectively. A. Position of induced barley mlo mutations and relative protein accumulation of the respective protein variants. Colored dots designate barley mlo mutant alleles as defined in additional file 1. The color code indicates protein variants with wild type-like accumulation (green), intermediate accumulation (yellow), and severely affected accumulation (red), while white indicates lack of data about protein accumulation of this variant. B. Position of site-directed mlo mutants and relative protein accumulation of the respective protein variants. Colored dots designate the site-directed barley mlo mutants generated in this (Figure 3) and previous studies [21, 22, 25]. Only mutant variants with at least 50% reduction in fungal entry rate are shown. The color code indicates protein variants with wild type-like accumulation (green), intermediate accumulation (yellow), and severely affected accumulation (red).