High-magnification CLSM detection of H
-DA at different developmental stages of the olive flower. A and B: projections of section stacks of the stigma surface at stages 1 and 3, respectively. The fluorescence localizes in association with the stigmatic papillae. C and D: optical section -and an enlarged view- of the stigmatic surface in an area lacking exudates at stage 4. E and F: optical section -and an enlarged view- of the stigmatic surface at stage 4. Green fluorescence extensively localizes in the exudate, as well as in stigmatic papillae and in small organelles inside some pollen grains (yellow arrows). G: projection of section stacks of the anther surface at stage 4. H: projection of section stacks of the stigma surface at stage 5. Fluorescence remains associated to the papillae and the pollen grains. Ex: exudate; n: nuclei; P: papillae; Pg: pollen grain; Pt: pollen tube.