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Figure 3 | BMC Plant Biology

Figure 3

From: The leader intron of AtMHX can elicit, in the absence of splicing, low-level intron-mediated enhancement that depends on the internal intron sequence

Figure 3

A 3-fold increase in expression level is seen in the context of the 35S promoter. A. Mean and standard errors of relative GUS activity in six biological replicates, each including an equal number of T2 seedlings from 20 independent transformants of each construct. GUS activity of the 35S construct was assigned the value of 1. B. Representative Northern blot results with the GUS probe. rRNA, ribosomal RNA. C. Mean and standard errors of relative GUS transcript levels in the six biological replicates. Quantification of band densities on gel was performed with the ImageJ program. The data are presented relative to the transcript level of the 35S construct.

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