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Figure 4 | BMC Plant Biology

Figure 4

From: The leader intron of AtMHX can elicit, in the absence of splicing, low-level intron-mediated enhancement that depends on the internal intron sequence

Figure 4

The modifications introduced in the different LIs. Boxed, partial sequence of the 5'UTR exons that border the LIs. Exon sequence was not altered in any of the constructs. The modifications introduced into the Wm and Wm-S constructs are highlighted in gray. The underlying numbers indicate the purpose of each modification: 1 - eliminating the main and potential cryptic splice sites, 2 - maintaining the length, deduced amino-acid sequence and predicted secondary structure of the natural uORF found in the 5'UTR of AtMHX [the first (ATG) and last (TGA) codons of the uORF of each construct are bold and underlined], 3 - creating SacII sites for obtaining the WmD-S from the Wm-S construct, 4 - eliminating the internal ATG codons, and 5 - eliminating a HindIII site that interfered with cloning into the binary vector.

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BMC Plant Biology

ISSN: 1471-2229

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