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Figure 6 | BMC Plant Biology

Figure 6

From: The leader intron of AtMHX can elicit, in the absence of splicing, low-level intron-mediated enhancement that depends on the internal intron sequence

Figure 6

The unspliced LI mediates low-level enhancement. A. Mean and standard errors of relative GUS activity in mixtures including an equal number of T2 seedlings from 13 independent transformants of each construct. GUS activity of the Wm construct was assigned the value of 1. EV, plants expressing the empty vector. The internal graph shows relative GUS activity compared to the Wm construct on a smaller scale in plants expressing the constructs that mediated low GUS activity. Statistical analysis (t-test) revealed a highly significant difference (p < 0.01) between plants expressing the Wm and all other constructs. A highly significant difference (p < 0.01) was also observed between plants expressing the -Int and the Wm-S constructs, but not between plants expressing the -Int and the WmD-S construct. B. Representative Northern blot results with the GUS probe. rRNA, ribosomal RNA.

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BMC Plant Biology

ISSN: 1471-2229

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