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Figure 2 | BMC Plant Biology

Figure 2

From: The Arabidopsis translocator protein (AtTSPO) is regulated at multiple levels in response to salt stress and perturbations in tetrapyrrole metabolism

Figure 2

Phenotype of mutants with different levels of AtTSPO expression. (A) Schematic representation of isolated insertional mutant of AtTSPO in Arabidopsis. Two copies of the T-DNA were inserted in tandem 123 bp upstream from the translational initiation codon of AtTSPO. (B) Total RNA was isolated from 5 day-old seedlings, reverse-transcribed and subjected to qRT-PCR. Data shown represent mean values obtained from independent amplification reactions (n = 3) and biological replicates (n = 2). Bars represent the standard error of biological replicates. (C) Root lengths of at least 100 individual 7-day-old seedlings grown in 16 h photoperiods. (D) Chlorophyll concentrations in 14-day-old, in vitro-grown plants of the indicated genotypes were determined spectrophotometrically. Values shown are means derived from three independent samples, each sample containing 100 mg of fresh weight. Units are μg of chlorophyll a + b per g of fresh weight (fw).

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