Proteomic identification of OsCYP2, a rice cyclophilin that confers salt tolerance in rice (Oryza sativa L.) seedlings when overexpressed

Table 1 Identification of shoot proteins of interest in hybrid rice by MALDI-TOF MS

Spot No.^a	Apparent MW (KD)/pI^b	MatchMW (KD)/pI^c	MOWSE Score^d	MOWSE Score for acceptance^e	No. MP^f	No. UMP^g	Percent covered^h	Accession No.^I	Protein name
P1	26.4/5.80	25.64/5.82	61	60	5	16	26	Q9FUE6	Putative glutathione S-transferase
P2	23.8/5.85	24.98/6.50	69	60	6	36	43	AAA57131	manganese superoxide dismutase
P3	24.2/5.91	23.555/5.81	62	60	4	17	28	Q84UH5	dehydroascorbate reductase
P4	52.5/6.05	52.688/5.85	92	60	8	11	28	NP_910282	putative phosphogluconate dehydrogenase
P5	65.5/6.5	71.06/6.54	61	60	6	15	16	Q6Z836	Putative L-aspartate oxidase
P6	17.5/6.32	18.682/6.28	84	60	6	33	49	XP_479920	putative cold shock protein-1
P7	36.2/6.95	30.783/6.99	80	60	5	26	31	CAE76006	Prohibitin
P8	18.5/9.21	18.319/8.61	117	60	10	4	42	AAA57046	Cyclophilin 2 (OsCYP2)
P9	10.2/9.65	22.566/9.8	117	60	7	4	37	XP_478627	Putative oxygen -evolving enhancer protein 3-1

^a Spot Nos refer to spot number as given in Figure 2. ^b Apparent MW (KD)/pI: apparent molecular weight and pI values. ^c MatchMW (KD)/pI: match molecular weight and pI values. ^d MOWSE Score: Scores given in MASCOT database. ^e MOWSE Score for acceptance: protein scores greater than 60 are significant (p < 0.05). ^f No. MP: number of matched peptides. ^g No. UMP: number of unmatched peptides. ^h Percent covered: percent of all match peptide sequences to OsCYP2 sequence. ^I Accession No.: Accession number in NCBI database.

ISSN: 1471-2229