Figure 5

Distribution of miRNAs in phloem exudate collected from different sites on the plant. Northern blot assays of 5 μg small RNA extracted from phloem exudate collected from base of the stem, pods and branches of L. albus plants. RNA samples were separated on a 15% denaturing polyacrylamide gel, transferred to Hybond-N⁺ nylon membrane and hybridized to specific ³²P end-labelled DNA oligonucleotide probes complementary to miR156, miR159, miR164, miR166, miR167, miR168, miR169, miR399 and miR395. Low molecular weight RNA was visualized by ethidium bromide staining to serve as loading control.