Figure 3

UGT73C6-YFP reporter generation and analysis. (a) Top: Adult phenotypes of independent transgenic lines expressing either a UGT73C6 _pro :UGT73C6-YFP construct (center) or a 35S _pro :UGT73C6-YFP construct (right) as compared to wild type (wt), grown for 4 weeks in long-day conditions (16 hrs 80-100 μmol·m^-2·s^-1 white light/8 hrs dark) at 21 ± 2°C. Bottom: Western blot analysis of UGT73C6-YFP protein levels in 2-week-old seedlings of the lines whose phenotype is shown above, using an anti-GFP antibody. (b) Representative YFP expression pattern of UGT73C6-YFP analyzed in leaves of eleven-day-old seedlings of line 35S _pro :UGT73C6-YFP/4 and line UGT73C6 _pro :UGT73C6-YFP/5 by fluorescence microscopy. The scale bars represent 10 μm.