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Table 1 Summary information of candidate gene (CG) sequences: Analyzed fragment length, gene coverage, number of lines, number of SNPs, rate ratio of non-synonymous to synonymous substitutions (d N /d S ), number of Indels and haplotypes, haplotype (Hd) and nucleotide diversity (Ï€), Tajima's D, and linkage disequilibrium (LD)

From: High levels of nucleotide diversity and fast decline of linkage disequilibrium in rye (Secale cerealeL.) genes involved in frost response

CG

Fragment length (bp)

Gene coverage a

No. of lines b

No. of SNPs c(non-synonymous)

d N /d S

No. of Indels

No. of haplotypes

Hd± SD

π± SD × 10-3(only exon)

Tajima's D

Intra-genic LD (r2)

ScCbf2

619

5'UTR/E

169

2 (0)

0.001

1

7

0.67 ± 0.02

1.5 ± 0.1 (1.4 ± 0.1)

1.17

0.13

ScCbf6

495

E

197

3 (0)

0.023

0

9

0.44 ± 0.04

3.6 ± 0.3

-0.35

0.77

ScCbf9b

1,371

5'UTR/E/3'UTR

183

30 (10)

0.174***

1

95

0.98 ± 0.03

7.1 ± 0.3 (11.5 ± 0.2)

1.71

0.14

ScCbf11

623

E

128

27 (12)

0.165

0

12

0.65 ± 0.02

14.5 ± 0. 9

1.74

0.51

ScCbf12

754

5'UTR/E/3'UTR

141

25 (8)

0.286***

1

48

0.89 ± 0.02

8.8 ± 1.0 (7.7 ± 0.1)

0.40

0.38

ScCbf14

560

E

185

5 (3)

0.606**

0

4

0.17 ± 0.04

1.5 ± 0.3

-0.27

0.92

ScCbf15

502

E

172

3 (3)

1.490***

1

9

0.68 ± 0.04

3.0 ± 0.2

2.14*

0.30

ScDhn1

435

5'UTR/E

138

4 (1)

0.128**

2

12

0.33 ± 0.05

2.7 ± 0.5 (4.4 ± 0.1)

-1.86*

0.48

ScDhn3

514

I/E/3'UTR

130

12 (2)

0.229***

2

21

0.73 ± 0.03

8.1 ± 0.6 (8.9 ± 0.1)

0.008

0.25

ScIce2

1,224

I/E

189

36d

n.a.

0

32

0.80 ± 0.02

11.2 ± 0.6 (0)

2.34*

0.36

ScVrn1

542

5'UTR/E

198

0

n.a.

1

2

0.11 ± 0.03

0.4 ± 0.1 (0)

-0.33

n.a.

Total

7,639

  

147 (39)

 

9

251

    
  1. a E: exon; UTR: untranslated region; I: intron
  2. b Failure of amplification in some of the lines may be due to the presence of SNPs/Indels in the binding sites of the sequences and/or the absence of some of the Cbf genes in some particular lines.
  3. c Minor allele frequency (MAF) > 0.05
  4. d SNPs are silent since they were all located in the first intron of the gene.
  5. Significance levels: * P < 0.05, ** P < 0.01, *** P < 0.001
  6. n.a.: not available