Figure 2

Phenotypic analysis of transgenic Arabidopsis plants ectopically expressing Actinidia flowering genes. A. Real-time RT-PCR analysis of transgenes in transgenic lines chosen for analysis. The expression of each gene was normalized against ACTIN. Error bars represent SE for three replicate reactions. B. Transgenic Arabidopsis plant expressing 35S::FUL-like (right) flowered earlier than the wild type plant (left) when grown in short day conditions. C. A compound terminal flower phenotype of 35S::FUL-like transgenic Arabidopsis. D. Wild type phenotype of flowers of a transgenic Arabidopsis plant expressing 35S::FUL. E. Transgenic Arabidopsis plant expressing 35S::SEP4 flowered early in long day conditions and produced smaller curled leaves. F. Wild type Arabidopsis grown as control for D. G. Transgenic Arabidopsis plant expressing 35S::AG (left) and grown in short days, flowered earlier than the wild type plant (right), after producing only four curled leaves. H. Protein interactions detected by yeast-two-hybrid assay. Yeast growth, representative of protein interaction, was classified as absent (-), weak (+/-), moderate (+) or strong (++). SEP4 bait (pDB-SEP4) was excluded from analysis due to strong auto-activation.