Figure 7
Involvement of OsMCA1 in the regulation of Trinitrophenol (TNP)-induced [Ca2+] cyt changes. (A) TNP-induced [Ca2+]cyt changes in cultured rice cells. Cells were treated with TNP (1 mM) at 0 min. A representative result of five experiments is shown. (B) Pharmacological analyses of TNP-induced [Ca2+]cyt changes. BAPTA (5 mM), GdCl3 (5 mM), LaCl3 (5 mM) and verapamil (1 mM) were added to the cell suspension 15 min prior to the TNP treatment; DMSO is used as the control. The peak intensities of aequorin chemiluminescence were compared; the relative luminescence level in the control was standardized as 100%. Data are the mean ± SE for three independent experiments. *P < 0.05; **P < 0.01; significantly different compared with control. (C) Effects of OsMCA1-suppression on TNP-induced [Ca2+]cyt changes. Cells were treated with TNP (1 μM). The peak intensities of aequorin chemiluminescence were compared; the relative luminescence level in the control line (20-5) was standardized as 100%. Data are the mean ± SE for three independent experiments. *P < 0.05; ***P < 0.005; significantly different compared with two control lines (20-5 and 5-3).