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Table 2 Sequencing statistics and mapping results of bulked samples using Maq-default and Maq-120 analysis

From: Combining SNP discovery from next-generation sequencing data with bulked segregant analysis (BSA) to fine-map genes in polyploid wheat

    

Maq-default analysis

Maq-120 analysis

 

Library

PE reads

Raw reads

raw reads mapping

%

reads in pairs

% 1

raw reads mapping

%

reads in pairs

% 1

High Bulk

400-bp

21,501,822

43,003,644

20,481,156

47.6%

17,044,066

83.2%

22,978,578

53.4%

19,080,072

83.0%

 

250-bp

31,917,705

63,835,410

22,992,792

36.0%

20,270,532

88.2%

26,489,852

41.5%

22,917,204

86.5%

 

Total

53,419,527

106,839,054

43,473,948

40.7%

37,314,598

85.8%

49,468,430

46.3%

41,997,276

84.9%

Low Bulk

400-bp

28,215,779

56,431,558

27,059,022

48.0%

22,074,113

81.6%

30,353,820

53.8%

24,719,488

81.4%

 

250-bp

32,981,686

65,963,372

25,389,354

38.5%

22,597,308

89.0%

29,236,028

44.3%

25,539,200

87.4%

 

Total

61,197,465

122,394,930

52,448,376

42.9%

44,671,421

85.2%

59,589,848

48.7%

50,258,688

84.3%

  1. 1 = (reads in pairs/raw reads mapping)*100