Combining SNP discovery from next-generation sequencing data with bulked segregant analysis (BSA) to fine-map genes in polyploid wheat

Table 4 Characteristics of the eight SNP markers surrounding the GPC-B1 locus

Unigene	Type¹	Informative base	12-fold²	16-fold²	Assay	Maq-default	Maq-120	Brachypodium	Rice	Sorghum	RPKM³	High Bulk	Low Bulk	BFR
Ta#S22380117	H	LDN	YES	YES	SSCP	YES	YES	3g03160	02g04330	04g002930	1.97	0.059	0.226	3.84
Ta#S18006314	S	-	NO	NO	SSCP	YES	NO	3g03270	02g04460	04g002980	51.72	0.255	0.832	3.27
Ta#S16259088	S	-	YES	YES	SSCP	YES	YES	3g03340	02g04500	04g003030	19.92	0.034	0.965	28.46
Ta#S37941845	H	LDN	YES	YES	KASPar	NO	YES	3g03330	02g04490	04g003020	29.92	0.130	0.861	6.60
Ta#S17984935	H	LDN	YES	YES	SSCP	YES	YES	-	02g04650	04g003150	28.85	0.075	0.436	5.84
Ta#S32700697	H	RSL65	YES	YES	KASPar	NO	YES	3g03530	02g04660	04g003160	19.36	0.397	0.017	23.00
Ta#S13163314	H	LDN	YES	YES	KASPar	NO	YES	1g39300	06g30910	10g020020	2.99	0.015	0.230	15.17
Ta#S17985476	H	RSL65	YES	YES	SSCP	YES	YES	3g03480	03g59470	01g004030	23.63	0.156	0.022	7.19

ISSN: 1471-2229