Figure 6

Expression analyses of OsHATs in rice. (A) RT-qPCR expression analyses of OsHATs in five different tissues: 23-h seed, seeds imbibed with water for 23 h; 4-day-old seedling, the whole plants of 4-day-old seedlings; and root, sheath and leaf tissues of two-leaf-stage seedlings. The relative amounts of mRNA for the eight OsHATs were measured. The data were expressed as the cycle number necessary to reach a threshold fluorescence value (Ct) and analyzed with the comparative Ct method (ΔΔCt). Expression values were normalized to those of eEF-1α and Ubq-1. Results are the average of three biological replicates, and each biological replicate consisted of three technical replications. Error bars represent standard errors. A different letter above each bar indicates a significant difference between tissues (p < 0.05, one-way ANOVA and LSD/SNK post hoc test). ( B- F) RT-qPCR analyses of OsHATs expression in rice leaves after treatment with hormones or abiotic stresses. The rice gene PR10a (known to be induced by ABA and SA) was used as a positive control. Expression was relative to Ubq-1 gene expression. Color bars, treated plants; white bars, untreated plants. According to Student’s t-test, * and ** indicate a significant difference between the treatment and the control at p < 0.05 and p < 0.01, respectively. (B) Treatment of two-leaf-stage seedlings with 100 μM ABA for 24 h. The seedlings were kept in water for the same duration as the control. (C) Treatment with 100 μM SA for 24 h or 36 h. (D) Treatment with 300 mM NaCl for 12 h. (E) Treatment with 4 ± 1 °C in the dark for 3 h. (F) Treatment with 42 °C for 3 h.