CYP98A22, a phenolic ester 3’-hydroxylase specialized in the synthesis of chlorogenic acid, as a new tool for enhancing the furanocoumarin concentration in Ruta graveolens

Table 1 Kinetic parameters of CYP98A22 determined with p- coumaroyl quinate and p -coumaroyl shikimate as substrate

Substrate	Product	V _max	K _m	V _i / K _m
		(pmol.min^-1.g^-1FW)	(μM)	(μM.pmol^-1.min^-1.g^-1FW)
p-coumaroyl quinate	caffeoyl quinate	0.49 +/- 0.16	7 +/- 5	0.07 +/- 0.02
p-coumaroyl shikimate	caffeoyl shikimate	0.19 +/- 0.03	24 +/- 7	0.008 +/- 0.002

Reactions were carried out at 28°C during 30 min in a KPi buffer 20Mm pH 7.0 as described in material and methods. Reactions were stopped with addition of 0.1% trifluoroacetic acid. Data obtained were fitted with the Michaelis-Menten equation.

ISSN: 1471-2229