Requirement of proline synthesis during Arabidopsis reproductive development

BMC Plant Biology

Table 1 Transmission of p5cs2 mutant alleles by self-pollination and in reciprocal crosses with wildtype plants

Genotype pollen donor¹	Genotype pollen recipient¹	Treatment	Offspring with *p5cs2*allele [% ±SD²]	Expected frequency³[%]	Seedlings analysed
A/a1	A/a1	none	58.9 ±1.3*	66.7	474
A/a1	A/a1	50 mM NaCl	64.1 ±0.5	66.7	457
A/a2	A/a2	none	59.1*	66.7	164
A/a2	A/a2	50 mM NaCl	68.2	66.7	176
A/A	A/a1	none	46.4	50	489
A/A	A/a2	none	40.8*	50	309
A/a1	A/A	none	46.1	50	371
A/a2	A/A	none	47.7	50	461

Transmission of the p5cs2 mutant alleles was determined by sulfadiazine (p5cs2-1) or BASTA (p5cs2-2) resistance of the progeny after selfing (first four rows) or reciprocal crossing of heterozygous p5cs2 mutants with wildtype plants.
¹ A: P5CS2 wildtype allele; a1: p5cs2-1 mutant allele; a2: p5cs2-2 mutant allele.
² SD was calculated from three batches of seeds from individual parent plants.
³ The expected frequency takes into account that homozygous p5cs2/p5cs2 seeds are usually non-viable in the presence of wildtype or heterozygous seeds in the same silique (compare Additional file 2 FigureS2).
* indicates significant difference from the expected value (p<0.05 by Χ² test with 1 degree of freedom).

ISSN: 1471-2229