A diploid wheat TILLING resource for wheat functional genomics

BMC Plant Biology

Table 1 Details of the primers and mutation frequencies found for genes in the T. monococcum TILLING population

Primer Name	Gene	Sequence 5′-3′	Number of mutants identified	Product size	No. of bases screened	Gene-wise mutation frequency
W1_F	waxy	TTGCTGCAGGTAGCCACAC	13	931	1141034	1/90 kb
W1_R		CTCAAGTGCTGCCTGGCAGAGAA
DOS_F		AGGACAACCAGCTACGCTTCAG	14	1058	1296685
DOS_R		TGATGACGTACCAGGAGAACGA
COMT1_AF	COMT1	CATGTACGCTCTCCAGCTCGTCTC	6	379	464502	1/86 kb
COMT1_AR		AGCTCTCCATGAGGACCTTGTC
COMT1_BF		GAACCACTCCATCATCATCACC	6	466	571130
COMT1_BR		CGAACTCCCTCTCGTACCTCTC
HCT2_AF	HCT2	CGCCGAGCGTCTACTTCTTC	4	308	377485	1/92 kb
HCT2_AR		GAGGAGCGGGTAGGAGGAGAT
HCT2_B		GGCCTGCACTTCATCAACTC	12	877	1074851
HCT2_BR		CGGTGCTTCTTTCTTTCTGC
4CL1_BF	4CL1	AGGACGCCTTCATGGCTAAGATCC	5	317	388515	1/100 kb
4CL1_BR		TCATGATCTGCTCGCCGCGGATGC
4CL1_CF		AGAGTCCACCAAGAACACCATC	7	701	859146
4CL1_CR		CTGGCTCTCAAGTCCTTCCTC

ISSN: 1471-2229