Figure 3

Microscopic analyses of fruit epidermal cells and pericarp sections during early fruit development. (A-C) Epidermal cells at the surface of immature green fruits (15 DPA). Photographs were taken with a Leica LDM 2500 microscope using a blue filter. (D-F) Paraffin transverse sections of pericarp tissues from MG fruits stained with Safranin O (a cell wall-specific dye). (G-I) Transverse sections of pericarp from MG fruits stained with Calcofluor White and visualized under UV light with a fluorescence microscope. Pc, parenchymatous cell; Cc, collenchymatous cell. The collapsed walls in parenchymatous cells of mesocarp tissues (D and G) are indicated by arrows. Sections were isolated from the same location in all fruits.