Figure 14

PCR confirmation of the genetic makeup of lpcat2 × dgat1 lines. Samples 1.1-1.5 (from WT, lpcat2, dgat1, and dgat1/lpcat2 H/H lines # 6-3-10-19 and # 6-3-20-1, respectively) were amplified using DGAT1 primers (Additional file 2: Table S2). Samples from dgat1, (1.3), dgat1/lpcat2 H/H lines # 6-3-10-19 (1.4) and # 6-3-20-1 (1.5), all have a 147 bp insert indicating they are homozygous for the insertion mutation resulting in a non-functional DGAT1 (as confirmed in Figure 2, above), while the WT (1.1) and lpcat2 (1.2) samples do not. T-DNA screening was performed on the samples of the same lines to test for the presence of LPCAT2. Samples 2.1-2.5 (same order as above) were amplified using SAIL_357_H01LP and SAIL_357_H01RP primers (Additional file 2: Table S2) and samples 3.1-3.5 (same order as above) were amplified using SAIL_357_H01RP and SAIL_LB2 primers (Additional file 2: Table S2). Collectively, sets 2.1-2.5 and 3.1-3.4 show that the lpcat2, and dgat1/lpcat2 H/H lines # 6-3-10-19 and # 6-3-20-1 samples have a homozygous knockout of the LPCAT2 gene.