Figure 5

Stability or mobilization of mPing in different selfed generations of the etoposide-treated plants of RZ1, revealed by DNA gel-blotting. (a) Complete stability of mPing was observed in eight randomly chosen etoposide-treated S0 plants. Also, no evidence for mobility was detected for a set of low-copy LTR retrotransposons (e.g., Osr35) in these S0 plants. (b) Mobilization of mPing was detected in at least 10 of the 19 randomly chosen individual plants of the S1 generation derived from a single S0 plant (S0-1). The empty and solid circles denote excision and reinsertion events, respectively. No evidence for mobility was detected for a set of low-copy LTR retrotransposons (e.g., Osr35) in any of these S1 plants. (c) Additional transposition of mPing was detected in 14 of the 15 S2 plants derived from a single S1 individual (S1-7) in which no transposition of this TE was detected. Only excisions were detected in these plants (marked by empty circles). Again, no evidence for mobility was detected for a set of low-copy LTR retrotransposons (e.g., Osr35) in any of these S2 plants. The DNAs in these blots were digested by XbaI.