Table 1 DNA methylation alterations of TEs and protein-encoding genes in the S0 generation of etoposide-treated plants relative to the controls, detected by methylation-sensitive gel-blotting in various rice genotypes
Probe | GenBank Accession No.a | DNA methylation changes in Etoposide-treated rice plants (S0) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Nipponbare | 93-11 | Matsumae | RZ1 | RZ2 | RZ35 | ||||||||
10 mg/L (H/M)b | 20 mg/L (H/M) | 10 mg/L (H/M) | 20 mg/L (H/M) | 10 mg/L (H/M) | 20 mg/L (H/M) | 10 mg/L (H/M) | 20 mg/L (H/M) | 10 mg/L (H/M) | 20 mg/L (H/M) | 10 mg/L (H/M) | 20 mg/L (H/M) | ||
Transposable elements (TEs) | |||||||||||||
Tos17 | AC087545 | n/- | n/- | n/n | n/n | n/n | n/n | n/n | n/n | n/- | n/n | n/- | n/n |
Osr2 | AL442110 | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n |
Osr23 | AP002843 | n/+ | n/+ | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n |
Osr35 | AC068924 | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n |
Osr36 | AP001551 | n/- | n/- | n/n | n/n | n/n | n/n | n/+ | n/+ | n/n | n/n | n/n | n/n |
Ping-specific | AB087616 | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n |
Pong-specific | BK000586 | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n |
mPing | AP005628 | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n |
Protein-encoding genes | |||||||||||||
Homeobox gene | AB007627 | n/- | n/- | n/n | n/n | n/n | n/n | n/- | n/n | n/n | n/n | n/n | n/n |
Binding | X88798 | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n |
CAL-2 | AK069341 | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n |
CAL-11 | X81393 | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n |
Elongation factor | D12821 | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n |
OsCDPK protein | AY144497 | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n | n/n |
CDPK-related protein kinase | AP004380 | n/n | n/n | n/n | n/n | n/n | n/n | +/+ | +/+ | n/n | n/n | n/n | n/n |
Variation frequency(%)c | 26.67 | 26.67 | 0 | 0 | 0 | 0 | 20 | 13.33 | 6.67 | 0 | 6.67 | 0 | |