Figure 6
Lesion development and induction of PAP5 following Botrytis cinerea infection. A, Size of lesion in wild type and pap5-1 plants inoculated after B. cinerea infection. Leaves were inoculated by placing 5 μl of the B. cinerea spore suspension (1 × 105/ml) on either side of the mid vein and the lesion size was measured after 3 days. The bars represent mean and SD of 20 individual lesions. Asterisks represent significant difference in lesion size compared to wild-type (Student’s t-test; P < 0.05). B, PAP5 expression in response to B. cinerea infection. Leaf tissues were harvested 48 h.p.i for RNA extraction. Transcript levels of PAP5 were normalized to the expression of GAPDH in the same samples. The transcript levels were expressed relative to the normalized transcript levels of infected wild-type plants.