Figure 3
From: The effect of Translationally Controlled Tumour Protein (TCTP) on programmed cell death in plants
45Ca overlay assay of recombinant AtTCTP. Ca2+-binding assay using radiolabelled calcium and recombinant AtTCTP. A shows a western blot of recombinant StrepII-tagged AtTCTP (MW = protein marker, AtTCTP = recombinant AtTCTP-StrepII; important marker band sizes are indicated). B shows the 45Ca overlay assay of AtTCTP, aequorin (aeq), RuBisCo (RBC), bovine serum albumin (BSA), and controls C1 (10 μl purified crude extract without StrepII-tag protein expression) and C2 (non Ca2+-binding, StrepII-tagged A. thaliana protein kinase). C shows Coomassie brilliant blue staining of the PVDF membrane used in 3B. If not indicated differently, 0.25 μg of each protein were spotted for 45Ca overlay assay.