Figure 3
From: The identification of novel loci required for appropriate nodule development in Medicago truncatula
The degree of rhizobial infection in Fix- mutants. Nodule sections of wild-type (A) and Fix- mutants (B-I). Nodules were harvested 3 weeks post inoculation with S. meliloti 1021 expressing the lacZ gene. 70 μm thick nodule sections were stained with X-gal to detect β-galactosidase activity. Nodules of (B) ipd3-1, (C) dnf5-2, (D) 7Y, (E) 5L, (F) 11S, (G) dnf8, (H) 13U and (I) dnf7-2 mutants show various degrees of bacterial colonization. Roman letters on wild-type nodules (A) indicate the zones of a mature nodule: I., meristem; II., infection zone; II-III., interzone; III., nitrogen-fixation zone. The arrow shows an infection thread in panel G. Bars represent 200 μm.