Figure 6

The location of Hsp17.6 _p :GFP expression maxima is temperature dependent. Hourly images of representative roots heat shocked for an hour at 28-37°C are shown. Arrowheads indicate the location of the brightest signal for the first two time points where the maximum is clear (either the differentiation zone in roots heat shocked from 28-37°C or the root tip in roots heat shocked at 40°C). Arrows indicate late vascular expression in roots heat shocked at 40°C. The contrast of all images in each temperature series was uniformly enhanced using the ImageJ 'enhance contrast’ tool so that both low and high expression patterns can be seen. As a result, intensity should not be compared between temperature series. Quantification of signal intensity can be found in Figure 5.