Figure 3

Time course of activation of LA by glyoxysomes from etiolated cucumber (A) or sunflower (B). β-oxidation assays were prepared as described in the material and method section with the only exception, that NAD⁺ was excluded from the assay. A: Activation of LA by glyoxysomes from cucumber. LA is directly activated to LA-CoA and converted to the 3-hydroxylated form. Small amounts of 13-HOD and 13-HOD-CoA are also observed. B: Activation of LA by glyoxysomes from sunflower. LA is rapidly oxidized to 13-HOD which is subsequently activated to 13-HOD-CoA and converted to 3-hydroxy-HOD-CoA. Small amounts of LA-CoA are also detected. HPLC-traces are shown at 234 nm, absorption signals of the individual intermediates are indicated by open (234 nm, conjugated double bond) or closed (260 nm, CoA-ester) circles.