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Figure 2 | BMC Plant Biology

Figure 2

From: The M3 phosphorylation motif has been functionally conserved for intracellular trafficking of long-looped PIN-FORMEDs in the Arabidopsis root hair cell

Figure 2

Phosphorylation-defective mutations in the M3 motif of PIN1 and PIN7 partly disrupted their PM localization in root hair cells. (A) and (B) Confocal images showing the subcellular localization of ProE7:PIN:GFP (WT-PIN), ProE7:3 m1-PIN:GFP (3 m1-PIN1), and ProE7:M3-PIN:GFP (M3-PIN) in root hair cells. Transgenic seedlings were observed with FM4-64 staining (2 μM, within 3 min). Representative images are shown. Bar = 10 μm in all cases. (C) Statistical analysis for the internalization of mutant PIN1 and PIN7 proteins. The ratios of root hair cells containing internally-localized PIN proteins were calculated with 29–36 total root hair cells from 10 seedlings for each transgenic line. Data represent means ± SE.

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BMC Plant Biology

ISSN: 1471-2229

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