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Table 1 Validated miRNA targets

From: Genome-wide characterization of microRNA in foxtail millet (Setaria italica)

Target ID miRNA ID

Target start-end

5′-target sequence-3′ complementary pattern 3′-miRNA sequence-5′

InterPro description of targets

  

↓1767(9/10)

B3 DNA binding domain Aux/IAA-ARF-dimerisation DNA-binding pseudobarrel domain AUX/IAA protein

Si005991m

1756-1776

5'-AGGCAUACAGGGAGCCAGGCA-3'

sit-miR160

 

3'-ACCGUAUGUCCCUCGGUCCGU-5'

  

↓1364(9/10)

AUX/IAA protein B3 DNA binding domain Auxin response factor Aux/IAA-ARF-dimerisation DNA-binding pseudobarrel domain

Si016509m

1353-1373

5'-AGCCAUACAGGGAGCCAGGCA-3'

sit-miR160

 

3'-ACCGUAUGUCCCUCGGUCCGU-5'

  

↓1328(6/10)

AUX/IAA protein B3 DNA binding domain Auxin response factor Aux/IAA-ARF-dimerisation DNA-binding pseudobarrel domain

Si034525m

1317-1337

5'-AGGCAUACAGGGAGCCAGGCA-3'

sit-miR160

 

3'-ACCGUAUGUCCCUCGGUCCGU-5'

  

↓1085(7/10)

Transcription factor GRAS

Si016508m

1074-1094

5'-GAUAUUGGCGCGGCUCAAUCA-3'

sit-miR171a

 

3'-CUAUAACCGCGCCGAGUUAGU-5'

sit-miR171b

 

3'-CUAUAACCGUGCCGAGUUAGU-5'

  

↓1082(2/10)

Transcription factor GRAS

Si016508m

1071-1091

5'-AGAGAUAUUGGCGCGGCUCAA-3'

nov-sit-miR15

 

3'-UCUCUAUAACCGCGCCGAGUU-5'

nov-sit-miR14

 

3'-CCUCUAUAACUGCGCCGAGUU-5'

nov-sit-miR49

 

3'-UCACUAUAACCGAGCCGAGU-5'

  1. Mapping of mRNA cleavage sites by RNA Ligase-Mediated 5′ RACE. The complementary pattern of miRNA sequences and partial sequences of the target mRNAs are shown in the table. All predicted mismatch base parings are shown in bold letters. The positions inferred as 5′ ends of miRNA-guided cleavage products, as revealed by 5′ RACE, and the numbers of sequenced 5′ RACE clones corresponding to each site are indicated by vertical arrowheads.
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BMC Plant Biology

ISSN: 1471-2229

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