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Table 1 RS activities in leaves of wild-type (Col-0) and RS5 mutants subjected to various abiotic stresses

From: Abiotic stress-induced accumulation of raffinose in Arabidopsis leaves is mediated by a single raffinose synthase (RS5, At5g40390)

 

4°C

WD

NaCl

MV

30°C

 

NS

S

NS

S

NS

S

NS

S

NS

S

Col-0

0.46 ± 0.046

1.86 ± 0.138

0.55 ± 0.014

1.17 ± 0.065

0.18 ± 0.005

0.34 ± 0.002

0.22 ± 0.004

0.33 ± 0.006

0.24 ± 0.004

0.33 ± 0.002

rs 5-1

0.15 ± 0.044

0.16 ± 0.028

0.16 ± 0.006

0.14 ± 0.002

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

rs 5-2

0.11 ± 0.061

0.12 ± 0.025

0.17 ± 0.009

0.16 ± 0.002

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

  1. Assays were conducted with desalted leaf crude extracts incubated with 100 mM Suc and 10 mM Gol at pH 7.5 for 1 h. NS, non-stressed; S, stressed (as per Materials and Methods); 4°C, cold-stressed; WD, water deficit-stressed; NaCl, salt-stressed; MV, methyl viologen-stressed; 30°C, heat-stressed. Values in bold represent increased RS activities in stressed as compared to non-stressed leaves. Values are the means ± SE of three independent replicates and represent RS activities expressed in nkat g-1 DW.
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BMC Plant Biology

ISSN: 1471-2229

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