Figure 6From: Interactions between ethylene and auxin are crucial to the control of grape (Vitis vinifera L.) berry ripeningEffect of Ethrel and AVG on auxin concentrations and the expression of selected auxin biosynthesis and GH3 genes in an ex planta experiment. IAA (A) and the IAA-amino acid conjugate, IAA-Asp, (B) were quantified by LC-MS/MS in ex planta pre-veraison Shiraz berries exposed to ReTain (125 mg L-1 AVG, 3% (w/v) sucrose), Ethrel (72 mg L-1 ethephon, 3% (w/v) sucrose), or Control (3% (w/v) sucrose) conditions for the indicated periods of time. FW, fresh weight. (C) Using the same tissues the expression of TAR1-TAR4, YUC1, GH3-1 and GH3-2 was analysed by qRT-PCR. Control, dark grey bars; Ethrel, light grey bars; AVG, white bars. Bars represent means ± SE (n = 3) and are denoted by a different letter if the means for each time point differ significantly (p < 0.05) using one-way ANOVA followed by Duncan’s post hoc test.Back to article page