Vinyl sulfone silica: application of an open preactivated support to the study of transnitrosylation of plant proteins by S-nitrosoglutathione

Begara-Morales, Juan C; López-Jaramillo, F Javier; Sánchez-Calvo, Beatriz; Carreras, Alfonso; Ortega-Muñoz, Mariano; Santoyo-González, Francisco; Corpas, Francisco J; Barroso, Juan B

doi:10.1186/1471-2229-13-61

Table 1 Summary of the positive identifications of the pea leaf proteins eluted from the GSNO vinyl sulfone resin

From: Vinyl sulfone silica: application of an open preactivated support to the study of transnitrosylation of plant proteins by S-nitrosoglutathione

Protein score C.I.%	Peptide count	Protein *species*	MW (kDa)	pI	ID UniProtKB	Comments
Chloroplastidic enzymes
		Fructose-1, 6- bisphosphatase			P46275	Benson-Calvin cycle enzyme.
100	9	with transit peptide	42.3	5.00		Regulated by thioredoxin.
100	9	mature protein	35.3			Reported as nitrated [88].
100	8	nicked protein	34.0	5.02		Reported as S-nitrosylated [43].
		Pisum sativum
100	11	Phosphoribulokinase	39.2	5.40	P93681	Benson-Calvin cycle enzyme.
100	11	Pisum sativum	39.2	5.40	P93681	Benson-Calvin cycle enzyme.
						Regulated by thioredoxin.
						Reported as nitrated [88].
		Ferredoxin: NADP reductase			P10933	It catalyzes the reduction of
100	9	with transit peptide	40.2	8.60		NADP⁺ to NADPH using the electrons provided by reduced ferredoxin.
100	8	mature protein	33.0	6.06
		Pisum sativum
						Reported as nitrated [89].
100	7	Hypothetical protein	42.8	8.19	Q9SA52	According to the UniprotKB:
		(predicted as chloroplastidic)				sugar epimerase family.
		Arabidopsis thaliana				Subcellular location: chloroplast.
Proteasome						Reported as S-nitrosylated [65].
100	6	26S proteasome alpha- 3 chain	27.5	6.11	O24362	Involved in the structure of the proteasome particle.
100	6	Spinacia oleracea	27.5	6.11	O24362	Involved in the structure of the proteasome particle.
100	8	Proteasome subunit alpha type- 7	27.2	6.86	Q9SXU1	Involved in the structure of the proteasome particle.
100	8	Cicer arietinum	27.2	6.86	Q9SXU1	Involved in the structure of the proteasome particle.
100	10	Proteasome subunit alpha type- 6	27.5	5.83	O48551	Involved in the structure of the proteasome particle.
100	10	Glycine max	27.5	5.83	O48551	Involved in the structure of the proteasome particle.
100	7	Proteasome subunit alpha type- 2- A	25.7	5.53	O23708	Involved in the structure of the proteasome particle.
100	7	Arabidopsis thaliana	25.7	5.53	O23708	Involved in the structure of the proteasome particle.
100	6	Putative beta4 proteasome subunit	14.6	6.40	Q93X32	Protease subunit.
		Nicotiana tabacum
Regulatory proteins
97.1	9	Putative F- box protein	38.1	9.80	Q9FVT4	F-box proteins have been associated to ubiquitination, signal transduction and regulation of the cell-cycle.
97.1	9	Arabidopsis thaliana	38.1	9.80	Q9FVT4
						Reported as nitrated [88].
98.8	8	GTP-binding protein	25.0	5.70	Q08147	Small GTP-binding proteins belong to a superfamily involved in the regulation as biotimers of a wide variety of cell functions.
98.8	8	Pisums sativum,	25.0	5.70	Q08147
						Reported as S-nitrosylated in mammals [62, 63].
99.7	12	CRK1 protein.	66.7	9.26	Q9LDC1	Cytokinine Regulated Kinase 1.
99.7	12	Beta vulgaris	66.7	9.26	Q9LDC1	Cytokinine Regulated Kinase 1.
98.8	11	Inducer of CBF expression 1 protein	60.8	6.51	Q1HIU3	C-repeat Binding Factor binds to CRT/DRE which actives many downstream genes that confer chilling and freezing tolerance to plants.
98.8	11	Populus suaveolens.	60.8	6.51	Q1HIU3
Others
100	15	Heat shock protein Hsp70	75.8	5.19	Q1SKX2	Hsp70s are part of the machinery for protein folding and protection from stress.
100	15	Medicago truncatula	75.8	5.19	Q1SKX2
						Reported as S-nitrosylated [37].
99.7	10	S- adenosyl- L- methionine:	41.6	6.21	Q9LS20	It catalyzes the formation of the methyl ester of the salicylic acid, involved in the localized and systemic defense responses of plants.
99.7	10	salicylic acid carboxyl methyltransferase	41.6	6.21	Q9LS20
		Arabidopsis thaliana

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ISSN: 1471-2229

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