Vinyl sulfone silica: application of an open preactivated support to the study of transnitrosylation of plant proteins by S-nitrosoglutathione

Begara-Morales, Juan C; López-Jaramillo, F Javier; Sánchez-Calvo, Beatriz; Carreras, Alfonso; Ortega-Muñoz, Mariano; Santoyo-González, Francisco; Corpas, Francisco J; Barroso, Juan B

doi:10.1186/1471-2229-13-61

Table 2 Summary of the positive identifications of the sunflower hypocotyls proteins eluted from the GSNO vinyl sulfone resin

From: Vinyl sulfone silica: application of an open preactivated support to the study of transnitrosylation of plant proteins by S-nitrosoglutathione

Protein score C.I.%	Peptide count	Protein *species*	MW (kDa)	pI	ID UniProtKB	Comments
		Ferredoxin- nitrite reductase	67.1	6.51	P05314	Involved in nitrogen metabolism, plant regeneration and morphogenesis.
99.8	12	precursor	67.1	6.51	P05314
100	14	fragment				Reported as nitrated [89].
100	14	Spinacia oleracea				Reported as nitrated [89].
97.6	9	Transcriptional adapter ADA2b	56.1	6.05	Q9ATB4	Component of transcriptional coactivator complexes. Importance in plant growth and development.
97.6	9	Arabidopsis thaliana	56.1	6.05	Q9ATB4
99.9	11	Expressed protein	43.0	5.72	Q2QWE0	Predicted.
99.9	11	Oryza sativa	43.0	5.72	Q2QWE0	Predicted.
99.9	10	At2g18860	34.0	5.87	Q501A1	Involved in endocytosis/exocytosis.
		syntaxin/t- SNARE protein.				Involved in endocytosis/exocytosis.
		syntaxin/t- SNARE protein.				Reported as S-nitrosylated [75].
		Arabidopsis thaliana				Reported as S-nitrosylated [75].
99.9	11	Pentatricopeptide repeat- containing protein- like protein	42.0	8.81	Q9CAY8	RNA-binding protein. Importance in RNA editing.
99.9	11	Arabidopsis thaliana	42.0	8.81	Q9CAY8	RNA-binding protein. Importance in RNA editing.
99.6	10	Hypothetical protein At2g46320	39.3	9.37	Q8S8M6	Mitochondrial carrier family.
99.6	10	Arabidopsis thaliana	39.3	9.37	Q8S8M6	Mitochondrial carrier family.
99.9	4	Pectinesterase	24.4	8.84	Q43838	It catalyzes the de-esterfication of pectin into pectate and methanol.
		Solanum tuberosum
						Involved in cell elongation.
						Reported as nitrated [88].
98.7	7	Catalase 1	34.2	5.9	Q93XK3	H₂O₂ scavenger enzyme.
		(fragment)				Inhibition by NO [79].
		Pinus pines				Inhibition by NO [79].
98	4	Thioredoxin peroxidase	30.1	8.2	Q8RVF8	H₂O₂ scavenger.
		Thioredoxin peroxidase				Some peroxiredoxin have been reported as S-nitrosylated [80].
		Nicotinum tabacum
99.7	6	Cytochrome P450	14.6	6.06	Q5EKR9	Biosynthetic reactions.
		(fragment)				NO reported to bind its Fe [87].
		Teucrium canadense				Reported as nitrated [89].
99.8	3	Calmodulin Arabidopsis thaliana	15.6	4.20	P25069	Ca binding protein Reported as nitrated [89].

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