Figure 2

Both osrecql4-1 and osrecql4-2 mutant plants show hypersensitivity to DNA damaging agents. Both osrecql4-1 and osrecql4-2 mutant plants exhibit significantly reduced root length compared to WT plants upon treatment with (A) 5 mg/L bleomycin (DSB inducer) or (B) 5 mg/L aphidicolin (S-phase arrestor). After germination, 5-day-old seedling were grown on MS medium containing bleomycin or aphidicolin. WT: DJ (Dongjin), NB (Nipponbare) and mutants segregated out: the total length of roots of osrecql4-1 (T-DNA), osrecql4-2 (Tos17) were measured. The results shown are from three independent repeats. From 10 to 15 seedlings were tested per treatment.